Effects of Delayed Fertilization on Viability
of Chinook Salmon Gametes.



Joseph F. Mickevich
School of Science and Natural Resources
Lake Superior State University
Sault Ste. Marie, MI 49783 USA




Abstract. Interval storage of Chinook Salmon (Oncorhynchus tshawytscha) gametes was
evaluated to examine the effects on fertilization and hatching success of embryos. Four
pair of male and female Chinook Salmon where taken from St. Mary's River in Sault Ste.
Marie, MI on 3 October, 1995. One hundred eggs where fertilized from each pair at eight
consecutive gamete storage time intervals. Percent survival was calculated for each pair
at each storage time interval and a Friedman Two-Way Analysis of Variance by Ranks
was used to test the data. Results show a decline in survival rates of Chinook Salmon
embryos, as storage time intervals increased, with a p-value < .05. A significant decline
in gamete viability took place after 36 hours of storage at 8.5 degrees Celsius, with zero
survival reported at 72 hours of storage.





Selected References


Munkittrik, K.R., S.M. McGeachy, M.G. Burke, P.A. Flett. 1992. Effects ofdelay in water
addition or rinsing on fertilization rates of Chinook Salmon, Coho Salmon, Atlantic Salmon,
and Rainbow Trout eggs. The Progressive Fish-Culturist. 54:14-20.

Piper R.G., l.B. McElwain, L.E. Orme, J.P. McCraren, L.G. Fowler, and J.R. Leonard. 1992.
Fish Hatchery Management. Fifth Printing. American Fisheries Society and United States
Fish and Wildlife Service. Washington, D.C.